Short Communication: Extraction of β-Casein from Goat Milk

Alphas1-casein, milk composition and coagulation properties of goat milk

Amounts of alphas1-casein (as1-CN), protein, fat, SNF and total solids were measured in 125 goat milk samples. Coagulation time, coagulation rate and curd ®rmness were measured in 75 goat milk samples by dynamic mechanical analysis using a Bohlin VOR Rheometer. After adjustments were made for month, time of milk collection and animal age, it was con®rmed that goat milk with high percent total s...

Detecting β-Casein Variation in Bovine Milk.

In bovine species, β-casein (β-CN) is characterized by genetic polymorphism. The two most common protein variants are β-CN A² (the original one) and A¹, differing from A² for one amino acid substitution (Pro67 to His67). Several bioactive peptides affecting milk nutritional properties can originate from β-CN. Among them, β-casomorphin-7 (BCM7) ranging from amino acid 60 to 66 can be released mo...

Expression of Human Serum Albumin in Milk of Transgenic Mice Using Goat β-casein/Human Serum Albumin Fusion Gene*

The gene encoding human serum albumin (HSA) was cloned from human liver cDNA library by PCR. The HSA cDNA in size of 2,176 bp, including 1,830 bp of open reading frame, was cloned into the plasmid carried with the 5’ flanking sequence of goat β-casein gene (-4,044 to +2,025 bp) to get a tissue specific expression vector in mammary gland named pGB562/HSA (12.5 kb). A 9.6 kb DNA fragment in which...

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus